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Comment on “RNA-guided DNA insertion with CRISPR-associated transposases”. Active Transposition of Insertion Sequences by Oxidative Stress in Deinococcus geothermalis. Chanjae Lee, Kyungsil Choo, Sung-Jae Lee.A Field Guide to Eukaryotic Transposable Elements. Mechanism and regulation of P element transposition. Elevated Temperatures Cause Transposon-Associated DNA Damage in C. elegans Spermatocytes. Detection of Genetic Elements Among Clinically Relevant Bacteria. CRISPR/dCas9‐mediated transposition with specificity and efficiency of site‐directed genomic insertions. Lena Goshayeshi, Sara Yousefi Taemeh, Nima Dehdilani, Mohammadreza Nasiri, Mohammad M.Targeted Conservative Cointegrate Formation Mediated by ISįamily Members Requires Sequence Identity at the Reacting End. ) offers an efficient genetic manipulation tool for vertebrates. Dan Shen, Chengyi Song, Csaba Miskey, Shuheng Chan, Zhongxia Guan, Yatong Sang, Yali Wang, Cai Chen, Xiaoyan Wang, Ferenc Müller, Zoltán Ivics, Bo Gao.CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering. Phosphorus Compounds of Natural Origin: Prebiotic, Stereochemistry, Application. The Structural, Functional and Evolutionary Impact of Transposable Elements in Eukaryotes. Dareen Almojil, Yann Bourgeois, Marcin Falis, Imtiyaz Hariyani, Justin Wilcox, Stéphane Boissinot.Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets. International Journal of Molecular Sciences 2021, 22 The Dynamism of Transposon Methylation for Plant Development and Stress Adaptation. Muthusamy Ramakrishnan, Lakkakula Satish, Ruslan Kalendar, Mathiyazhagan Narayanan, Sabariswaran Kandasamy, Anket Sharma, Abolghassem Emamverdian, Qiang Wei, Mingbing Zhou.Unbiased profiling of CRISPR RNA-guided transposition products by long-read sequencing. Probabilistic identification of bacterial essential genes via insertion density using TraDIS data with Tn5 libraries. Valentine U Nlebedim, Roy R Chaudhuri, Kevin Walters.Detection of the Enzymatic Cleavage of DNA through Supramolecular Chiral Induction to a Cationic Polythiophene. Trévisan, Valentine Cyriaque, Ruddy Wattiez, David Beljonne, Sébastien Richeter, Sébastien Clément, Mathieu Surin. Oligomerization of THAP9 Transposase via Amino-Terminal Domains. This article is cited by 32 publications. In fact, for many applications, the almost-perfect randomness and high frequency of integration make transposon-based approaches indispensable. Although several non-transposon-based approaches to site-directed genome modifications have emerged in the past decade, transposon-based applications are highly relevant when integration specificity is not sought. Therefore, a second focus of the article is to provide a nonexhaustive overview of transposon applications. The DD(E/D) group, in addition to being the largest and most common among all DNA transposases, is the one whose members have been used for a wide variety of genomic applications. For these, a number of three-dimensional structures of transpososomes (transposase–nucleic acid complexes) are available, and we use these to describe the basics of their mechanisms.
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From a mechanistic perspective, we have focused on one particular group of DNA transposons that encode a transposase with a DD(E/D) catalytic domain that is topologically similar to RNase H. Here, we first provide an overview of the classification of such mobile elements in a variety of organisms. Movement is facilitated by one or more proteins, called the transposase, typically encoded by the mobile element itself. DNA transposons are defined segments of DNA that are able to move from one genomic location to another.